An Isolation Method of Protein from Single Cell by Impact-Cell-Mill Part II. Destruction of single cell microbes

An experiment to isolate protein from single cell was carried to obtain the following results. Under the condition of spouting pressure 600kg/cmcm2, clearance between nozzle and disk 30mm, petroleum yeast and Chlorella algae could be crashed about 98%, Baker's Yeast could be done about 93%. The most suitable clearance betwen nozzle and disk to crash single cell was 20 mm at spouting pressure 400kg/cmcm2, and 30mm at over 500 kg/cmcm2. When the concentration of the suspension of single cells were high, it's viscosity showed above 150cp, and Impact-Cell-Mill could not show high pressure to envelopm any small air bladder cells resulting in the reduction of the destruction ratio of single cell. (Received May. 24, 1971)